Description
For quantitative detection of mouse Meteorin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Meteorin, glial cell differentiation regulator is a protein that in humans is encoded by the METRN gene. The mouse Metrn gene is mapped to chromosome 17. It is concluded that meteorin acts directly on satellite glial cells to induce a change in morphology and that transformed satellite glial cells induce the secondary effect of neurite extension. In cerebellar explants, meteorin induced conversion of cerebellar astrocytes into radial glia and promoted astrocyte differentiation from embryonic day 11.5 cerebral cortex neurospheres.
Principle: Mouse Meteorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for Meteorin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G22-D291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Meteorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Meteorin amount of sample captured in plate